Isolation and Characterization of Quercetin Glycosides and Antioxidant Phytochemicals from Azadirachta indica Leaf Extract
Abstract
Neem (Azadirachta indica) is consumed as vegetable in some parts of Southeast Asia, and as medicine and insecticides. This study reports the quantitative analysis of the major and minor antioxidant phytochemicals from its leaves, isolation and characterization process of them. Antioxidant activities were characterized by the ability of reducing power, DPPH scavenging, hydroxyl radicals scavenging and hydrogen peroxide scavenging. All antioxidant assay results showed that ethyl acetate fraction exhibited the strongest antioxidant activities and the highest total phenol content. Two major compounds (quercetin-3-O-galactoside and quercetin-3-rhamnoside) and four minor compounds (ferulic acid, myricetin, kaempferol and quercetin) were isolated and identified from the ethyl acetate extract using silica gel and Sephadex LH-20 column chromatography. The structures of the major compounds were identified from UV-Vis and LC-MS-MS chromatograms.
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